[Retracted] Mild Hypothermia Promotes Ischemic Tolerance and Survival of Neural Stem Cell Grafts by Enhancing Global SUMOylation

Figure 2

Effect of mild hypothermia (33°C) on hypoxic NSCs. (a) LDH contents of the control (37°C) and mild hypothermia groups were measured by ELISA. (b) Apoptosis assays were measured by flow cytometry. Data indicate (). (c) Expression of NSE or nestin (red fluorescent signal) and GFAP (green fluorescent signal) in the control NSCs or mildly hypothermic and hypoxic NSCs was examined by immunofluorescence; DAPI (blue) was used to stain cell nuclei (scale bar, 10 μm). (d) Oct4 and SOX2 protein expression was examined by western blotting. Data shown indicate (). (e) ECAR analysis of the glycolytic capacity of the control NSCs or mildly hypothermic and hypoxic NSCs. Data shown indicate (); and compared with 37°C group.

(a)

(b)

(c)

(d)

(e)