[Retracted] Mild Hypothermia Promotes Ischemic Tolerance and Survival of Neural Stem Cell Grafts by Enhancing Global SUMOylation

Figure 4

Overexpression of UBC9 increased NSC stemness and tolerance to hypoxia. (a) and (b) Expression of SUMO1 and SUMO2/3 conjugates, free SUMO1 and SUMO2/3, UBC9, Oct4, and SOX2 in NSCs overexpressing UBC9, as assessed by western blotting. (c) Quantitative data were normalized to GAPDH and expressed as (). (d) Expression of NSE or nestin (red fluorescent signal) and GFAP (green fluorescent signal) in NSCs of the UBC9 transgene and Con groups after 12 h hypoxia was examined by immunofluorescence; DAPI (blue) was used to stain cell nuclei (scale bar, 10 μm). (e) LDH contents were measured by ELISA, and data shown indicate (). (f) Western blotting to detect cleaved caspase-3 expression in NSCs. Quantitative analysis: cleaved caspase-3 expression was normalized to GAPDH and is expressed as (). and vs. control.

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