The Role of CD147 in Pathological Cardiac Hypertrophy Is Regulated by Glycosylation

Figure 6

CD147 glycosylation regulated CD147-TRAF2 binding and subsequent activation of TRAF2-TAK1 signalling. (a) TRAF2 binding with CD147-OE and CD147-mutant was observed under physical conditions (). Flag-tagged CD147-OE or CD147-mutant were expressed in mouse hearts. Proteins interacting with CD147 in tissue lysate were immunoprecipitated with anti-DYKDDDDK IP Resin and analysed by western blot using an anti-TRAF2 antibody. (b) Representative image of immunoprecipitation and (c) quantitative analysis of the recruitment of TRAF2 to CD147-OE and CD147-mutant proteins in response to TAC (). (d) Representative western blots and quantitative results of phosphorylated and total TAK1 protein levels in hearts from the indicated mouse groups after TAC (). (e) Representative western blots and quantitative results for phosphorylated and total protein levels of p38, AMPK, AKT, and glycogen synthase kinase 3β (GSK3β) in hearts from the different mouse groups after TAC (). vs. the sham group; vs. the sham group; vs. the sham group; vs. the sham group; ^# vs. the vehicle group; ^## vs. the vehicle group; ^✟ vs. the OE group; ^✟ vs. the OE group.

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