Research Article

NOL12 as an Oncogenic Biomarker Promotes Hepatocellular Carcinoma Growth and Metastasis

Figure 7

NOL12 knockdown inhibited cell proliferation of HCC cells in vitro and in vivo. The expression levels of NOL12 in 12 pairs of fresh adjacent nontumor tissues and liver cancer tissues (a) and HCC cell lines (b) were detected by RT-qPCR. (c) Endogenous NOL12 proteins were efficiently silenced (shNOL12-1, shNOL12-2) in Huh-7 and HepG2 cells. Actin was used as control. (d, e) MTT assays were performed to determine the proliferation of HCC cells transduced with the NOL12 shRNA lentivirus. (f, g) Cell colony formation assays were performed in HCC cells. (h) Tumor volume was monitored in xenograft samples from stable NOL12 knockdown Huh-7 cells or shCtrl. (i) Final weight of tumor masses obtained from stable NOL12 knockdown Huh-7 cells or shCtrl was compared. (j) The proportion of Ki67-expressing cells in xenograft tumors was examined by immunohistochemistry. , , and .