The Combination of Rhodosin and MMF Prolongs Cardiac Allograft Survival by Inhibiting DC Maturation by Promoting Mitochondrial Fusion

Figure 3

Rho and combination medication treatment increases the proportion of CD4⁺CD25⁺Foxp3⁺ T cells in CD4⁺T cells. (a) CD4 lymphocyte isolated from mice spleens treated with Rho and combination of Rho with low dose of MMF was shown to contain a higher proportion of Tregs (CD4⁺CD25⁺Foxp3⁺) than saline-treated Controls. (b) Percentage of Tregs (CD4⁺CD25⁺Foxp3⁺) in draining lymph nodes was significantly increased in Rho-treated and combination treatment mice in comparison with controls on isolation on day 5 after full MHC-mismatch cardiac transplantation (representative flow cytometry plots, pregated on CD4⁺ cells.). (c, d) The quantitative analysis of the Treg cells in CD4⁺ T cells in the spleens and draining lymph nodes by GraphPad Prism 8.0. Data of histograms are presented as from six independent experiments (, , mice per group versus the control group).