Fucoxanthin Attenuates Oxidative Damage by Activating the Sirt1/Nrf2/HO-1 Signaling Pathway to Protect the Kidney from Ischemia-Reperfusion Injury

Figure 3

Fucoxanthin attenuated ROS levels and apoptosis in HK-2 cells after H/R injury. The H/R model was established by hypoxia for 12 hours and reoxygenation for 6 hours. The HK-2 cells were treated with fucoxanthin (0.1 μM, 1 μM, and 5 μM) for 24 hours and then experienced H/R. (a) Cytotoxicity of fucoxanthin on HK-2 cells. (b) and (c) SOD1 protein expression. (d)–(e) Representative images of DCFH-DA staining (magnification ×100; ) and their quantitative analysis. (f)–(i) Expression of apoptosis-related proteins Bcl-2, Bax, cleaved caspase-3, and their quantitative analysis. (j) and (k) Percentage of apoptotic cells detected by flow cytometry. Values are expressed as , . ^# compared with control group, and compared with group.

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