Research Article

miR-154-5p Affects the TGFβ1/Smad3 Pathway on the Fibrosis of Diabetic Kidney Disease via Binding E3 Ubiquitin Ligase Smurf1

Figure 6

Smurf1 regulates ubiquitination through Smad3. CCK-8 cell proliferation (a) and related protein Smurf1, pSmad3/Smad3, and ubiquitin concentration (b). Co-IP verification for the combination of Smurf1 and Smad3 (c) after pretreatment with Smurf1 siRNA and TGFβ1/Smad3 inhibitor, SB431542. vs. Group 1, ; #vs. Group 2, ; &vs. Group 3, . samples/group.