|
| Names | Information |
|
| Treatment |
| Rno-miR-154-5p mimic forward | 5 - UAG GUU AUC CGU GUU GCC UUC G -3, GenePharma |
| Rno-miR-154-5p mimic reverse | 5 - AAG GCA ACA CGG AUA ACC UAU U -3, GenePharma |
| Rno-miR-154-5p inhibitor | 5 - CGA AGG CAA CAC GGA UAA CCU A -3, GenePharma |
| Rno-miR-negative control | 5 - CAG UAC UUU UGU GUA GUA CAA -3, GenePharma |
| Smurf1 siRNA forward sequence | 5 - CAU AUC GCC AGA UCA UGA ATT -3, GenePharma |
| Smurf1 siRNA reverse sequence | 5 - UUC AUG AUC UGG CGA UAU GTT -3, GenePharma |
| Negative control siRNA forward | 5 - UUC UCC GAA CGU GUC ACG UTT -3, GenePharma |
| Negative control siRNA reverse | 5 - ACG UGA CAC GUU CGG AGA ATT -3, GenePharma |
| TGFβ1/Smad3 inhibitor, SB431542 | #14775, 10 nM for 24 h, dissolved in DMSO, Cell Signaling Technology |
| Detection |
| Rno-miR-154-5p probe | 5-FAM-CGA AGG CAA CAC GGA TAA CCT A-FAM-3 for FISH, GenePharma |
| Rno-miR-154-5p primer forward | 5 - CTG CCG TAG GTT ATC CGT G -3, GenePharma |
| Rno-miR-154-5p primer reverse | 5 - AGA GCA GGG TCC GAG GAT -3, GenePharma |
| U6 primer forward | 5 - CTC GCT TCG GCA GCA CA -3, GenePharma |
| U6 primer reverse | 5 - AAC GCT TCA CGA ATT TGC GT -3, GenePharma |
| TGFβ1 primary antibody | Rabbit monoclonal antibody, ab215715, 44 kDa, 1 : 1000 for WB. Abcam |
| Smad3 primary antibody | Rabbit antibody, #9523, 52 kDa, 1 : 1000 for WB, 1 : 100 for IP, Cell Signaling Technology |
| pSmad3 primary antibody | Rabbit antibody, #9520, 52 kDa, 1 : 1000 for WB, Cell Signaling Technology |
| Smurf1 primary antibody | Mouse monoclonal antibody, sc-100616, 86 kDa, 1 : 200 for WB, 2 μg/100 μg total protein for IP, Santa Cruz Biotechnology |
| Ubiquitin primary antibody | Rabbit antibody, #3933, full bands, 1 : 1000 for WB, Cell Signaling Technology |
| β-Actin primary antibody | Rabbit antibody, #4970, 45 kDa, 1 : 1000 for WB, Cell Signaling Technology |
| IgG primary antibody | Rabbit antibody isotype control, #3900, for IP, Cell Signaling Technology |
| IgG primary antibody | Mouse antibody isotype control, #5415, for IP, Cell Signaling Technology |
| DAPI | For nucleus, #4083, for IF, Cell Signaling Technology |
| Anti-rabbit IgG | Anti-rabbit, #3678, for WB, Cell Signaling Technology |
| Anti-mouse IgG-HRP secondary antibody | Anti-mouse, #7076, for WB, Cell Signaling Technology |
| Anti-rabbit IgG-HRP secondary antibody | Anti-rabbit, #7074, for WB, Cell Signaling Technology |
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