|
Names | Information |
|
Treatment |
Rno-miR-154-5p mimic forward | 5 - UAG GUU AUC CGU GUU GCC UUC G -3, GenePharma |
Rno-miR-154-5p mimic reverse | 5 - AAG GCA ACA CGG AUA ACC UAU U -3, GenePharma |
Rno-miR-154-5p inhibitor | 5 - CGA AGG CAA CAC GGA UAA CCU A -3, GenePharma |
Rno-miR-negative control | 5 - CAG UAC UUU UGU GUA GUA CAA -3, GenePharma |
Smurf1 siRNA forward sequence | 5 - CAU AUC GCC AGA UCA UGA ATT -3, GenePharma |
Smurf1 siRNA reverse sequence | 5 - UUC AUG AUC UGG CGA UAU GTT -3, GenePharma |
Negative control siRNA forward | 5 - UUC UCC GAA CGU GUC ACG UTT -3, GenePharma |
Negative control siRNA reverse | 5 - ACG UGA CAC GUU CGG AGA ATT -3, GenePharma |
TGFβ1/Smad3 inhibitor, SB431542 | #14775, 10 nM for 24 h, dissolved in DMSO, Cell Signaling Technology |
Detection |
Rno-miR-154-5p probe | 5-FAM-CGA AGG CAA CAC GGA TAA CCT A-FAM-3 for FISH, GenePharma |
Rno-miR-154-5p primer forward | 5 - CTG CCG TAG GTT ATC CGT G -3, GenePharma |
Rno-miR-154-5p primer reverse | 5 - AGA GCA GGG TCC GAG GAT -3, GenePharma |
U6 primer forward | 5 - CTC GCT TCG GCA GCA CA -3, GenePharma |
U6 primer reverse | 5 - AAC GCT TCA CGA ATT TGC GT -3, GenePharma |
TGFβ1 primary antibody | Rabbit monoclonal antibody, ab215715, 44 kDa, 1 : 1000 for WB. Abcam |
Smad3 primary antibody | Rabbit antibody, #9523, 52 kDa, 1 : 1000 for WB, 1 : 100 for IP, Cell Signaling Technology |
pSmad3 primary antibody | Rabbit antibody, #9520, 52 kDa, 1 : 1000 for WB, Cell Signaling Technology |
Smurf1 primary antibody | Mouse monoclonal antibody, sc-100616, 86 kDa, 1 : 200 for WB, 2 μg/100 μg total protein for IP, Santa Cruz Biotechnology |
Ubiquitin primary antibody | Rabbit antibody, #3933, full bands, 1 : 1000 for WB, Cell Signaling Technology |
β-Actin primary antibody | Rabbit antibody, #4970, 45 kDa, 1 : 1000 for WB, Cell Signaling Technology |
IgG primary antibody | Rabbit antibody isotype control, #3900, for IP, Cell Signaling Technology |
IgG primary antibody | Mouse antibody isotype control, #5415, for IP, Cell Signaling Technology |
DAPI | For nucleus, #4083, for IF, Cell Signaling Technology |
Anti-rabbit IgG | Anti-rabbit, #3678, for WB, Cell Signaling Technology |
Anti-mouse IgG-HRP secondary antibody | Anti-mouse, #7076, for WB, Cell Signaling Technology |
Anti-rabbit IgG-HRP secondary antibody | Anti-rabbit, #7074, for WB, Cell Signaling Technology |
|