Sesamol Attenuates Neuroinflammation by Regulating the AMPK/SIRT1/NF-κB Signaling Pathway after Spinal Cord Injury in Mice

Figure 3

Sesamol attenuates inflammation and induces M2 phenotype microglial polarization in mice suffering from SCI. (a) Western blot images showing protein levels of IL-6, TNF-α, CD86, Arg-1, and CD206 in the injured spinal cord of mice at 3 dpi. (b, c) Quantification of TNF-α and IL-6 from (a), respectively (). (d, e) Statistical analysis of IL-6 and TNF-α concentrations determined by ELISA in BV2 cells suffering from LPS ( per group). (f–h) The quantitative analysis of CD86, CD206, and Arg-1 from (a) ( per group). (i) Sesamol increased the fluorescence degree of CD206 (green) in activated microglia (established by IBA1, red), and DAPI (blue) is for the nuclear staining (). Values are represented as the . ^#, ^##, ^###, and ^####.

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