Clinical Relevance and Tumor Growth Suppression of Mitochondrial ROS Regulators along NADH:Ubiquinone Oxidoreductase Subunit B3 in Thyroid Cancer

Figure 5

Overexpression of NDUFB3 or sideroxylin suppresses thyroid tumor growth in vivo. (a) A sketch map showing mice bearing thyroid tumors derived from BCPAP or C643 cells stably overexpressing NDUFB3 or its empty control vector in dorsal tissues. (b) Tumor volumes over the indicated times were analyzed. Mice were euthanized as their tumor volumes reached approximately 3000 mm³. Each line represents a mouse, and each plot on the line represents a different time point. values represent two-way ANOVA and post hoc Tuckey’s tests for tumor growth. ; ; . (c) Survival curves of tumor-bearing mice overexpressing NDUFB3 or empty vector controls. Analysis was statistically evaluated using the log-rank test. (d) Representative flow cytometry analysis of single-cell suspensions from resected tumor masses (vector versus NDUFB3). (e) A sketch map showing mice bearing thyroid tumors derived from BCPAP or C643 cells stably expressing shRNA against NDUFB3 or its empty control vector in dorsal tissues. (d) Tumor volumes over the indicated times were analyzed. Mice were euthanized as their tumor volumes reached approximately 3000 mm³. Each line represents a mouse, and each plot on the line represents a different time point. values represent two-way ANOVA and post hoc Tuckey’s tests for tumor growth. ; . (e) The survival curves of tumor-bearing mice with NDUFB3 knockdown or its empty vector controls. Analysis was statistically evaluated using the log-rank test. (f) Representative flow cytometry analysis of single-cell suspensions from resected tumor masses (shNDUFB3 versus shCtrl). (g) A sketch map showing that mice bearing thyroid tumors were treated with sideroxylin or vehicle after BCPAP cells or C643 cells were inoculated into the dorsal tissues of mice. (h) Tumor volumes over the indicated times were analyzed. Mice were euthanized as their tumor volumes reached approximately 3000 mm³. Each line represents a mouse, and each plot on the line represents a different time point. values represent two-way ANOVA and post hoc Tuckey’s tests for tumor growth. ; ; . (i) Survival curves of tumor-bearing mice treated with sideroxylin or vehicle. Analysis was statistically evaluated using the log-rank test. (j) Representative flow cytometry analysis of single-cell suspensions from resected tumor masses (vehicle versus sideroxylin).

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