Research Article

Interaction between TRPML1 and p62 in Regulating Autophagosome-Lysosome Fusion and Impeding Neuroaxonal Dystrophy in Alzheimer’s Disease

Figure 5

TRPML1/p62 mediates the BDNF/TrkB signaling pathway and axonal development in vitro. (a) Interaction between TRPML1 and p62 assessed by FRET. (b) Protein expression of BDNF and the ratio of p-TrkB/TrkB and p-CREB/CREB in neurons, evaluated by Western blot analysis. (c) BDNF nuclear translocation in neurons with alternative transfection detected by immunofluorescence staining. (d) Length of axons in neurons observed under an inverted microscope. Measurement data were described as the . One-way ANOVA was used for comparisons among multiple groups, followed by Tukey’s post hoc test. The cell experiment was repeated 3 times independently. vs. untreated HT22 cells; ^ vs. cells treated with sh-NC; # vs. cells treated with oe-NC; & vs. cells treated with oe-TRPML1.
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