Research Article

Suppression of Selective Voltage-Gated Calcium Channels Alleviates Neuronal Degeneration and Dysfunction through Glutathione S-Transferase-Mediated Oxidative Stress Resistance in a Caenorhabditis elegans Model of Alzheimer’s Disease

Figure 5

Calcium acetate aggravated Aβ aggregation by increasing oxidative stress in Aβ worm, while EGTA and nimodipine had opposite effects. (a‑e) Representative images of Aβ deposits in N2, CL2355, and CL2355 worms treated with calcium acetate, EGTA, and nimodipine (scale bar, 20 μm). The area in the red rectangle was observed. Arrows indicate ThS staining. (f) Histogram of the mean number of Aβ deposits in the head region/anterior area of the worms. (g–k) Representative images of ROS content tested by the dihydroethidium (DHE) red fluorescence, in N2, CL2355, and CL2355 worms treated with calcium acetate, EGTA, and nimodipine (scale bar, 100 μm). (l) Histogram of the mean ROS content. Error bars indicate the SEM. (m) Histogram of the mean ATP content in N2, CL2355, and CL2355 worms treated with calcium acetate, EGTA, and nimodipine. ( vs. control, vs. control).
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