Electroacupuncture Attenuated Anxiety and Depression-Like Behavior via Inhibition of Hippocampal Inflammatory Response and Metabolic Disorders in TNBS-Induced IBD Rats

Figure 5

EA at ST36 and SP6 blocked the activation of TLR4/NF-κB signaling pathway and NLRP3 inflammasomes (/group). Representative images of immunofluorescence staining of hippocampal slices for DAPI (blue, nuclei indicator), , , P-NF-κB p65 (red), and IL-1β (green). Original magnification ×400. (a1) and (a2) The immunofluorescence staining and histogram of . (b1) and (b2) The immunofluorescence staining and histogram of . (c1)–(c3) The immunofluorescence staining and histogram of P-NF-κB p65 and the histogram of NF-κB p65. (d1) and (d2) The immunofluorescence staining and histogram of IL-1β. (e) The histogram of ZO-1. (f) The histogram of MyD88. (g) The histogram of TAK1. (h) The histogram of p38 MAPK. (i) The histogram of P-p38 MAPK. Data were shown as . and by Bonferroni’s post hoc test.

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