Research Article

Pioglitazone Hydrochloride Extends the Lifespan of Caenorhabditis elegans by Activating DAF-16/FOXO- and SKN-1/NRF2-Related Signaling Pathways

Figure 2

The effect of PGZ on lifespan extension depends on the IIS pathway. (a–d) Lifespan analyses of nhr-49(gk405) (a), daf-2(e1370) (b), akt-1(mg144) (c), and daf-16(mu86) (d) mutant worms treated with or without 0.5 mM PGZ ( value by the log-rank test). (e) mRNA levels of daf-16 target genes (sod-1, sod-3, ctl-1, and dod-3) in WT animals with or without PGZ treatment (; ; and ; Student’s -test). (f) Western blot analyses of SOD-3p::GFP in worms treated with or without 0.5 mM PGZ. Actin was shown as the loading control. (g) Imaging of fluorescence in the transgenic strain CF1553 (SOD-3p::GFP) (representative of three experiments) and (h) respective quantitative results for at least 30 worms per condition (; ; ; Student’s -test).
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