Low-Temperature Plasma-Activated Medium Inhibited Proliferation and Progression of Lung Cancer by Targeting the PI3K/Akt and MAPK Pathways

Figure 3

(a) Schematic representation of the experiment workflow. (b) Bright field images of 2D and 3D culture lung adenocarcinoma cells (A549 and H23) and lung squamous carcinoma cells (SK-MES-1 and H520) at day 6; scale bar, 100 μm. (c) ROS accumulation in PAM-treated 2D cells and 3D spheroids; scale bar, 100 μm. Nontreated cells and spheroids as control. (d) Quantification of the relative intracellular ROS level in 2D cells and 3D spheroids. (e) Kinetic analysis of the intracellular ROS level in 2D cells and 3D spheroids measured at different time intervals following PAM treatment.

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