Long Noncoding RNA SCIRT Promotes HUVEC Angiogenesis via Stabilizing VEGFA mRNA Induced by Hypoxia

Figure 3

SCIRT increases the angiogenesis via stabilizing VEGFA mRNA under HR condition. (a) SCIRT upregulated VEGFA with HR treated in HUVECs, which was inhibited when cells transfected with siRNA targeting VEGFA. Protein levels were determined by western blot. (b) Tube formation assay, transwell migration assay, and wound healing assay were employed to evaluate the angiogenesis ability of HUVECs, which was increased with ectopic expression of SCIRT, while being repressed with the inhibition of VEGFA (scale bar, 100 μm). Quantitative analyses of tube formation assay, wound healing assay, and transwell migration assay are shown in (c–e), respectively. (f) Inhibition of SCIRT downregulates VEGFA mRNA expression. (g) No alterations of VEGFA mRNA level were observed as ectopic expression of SCIRT. (h) Cells were treated with ActD and harvested at various time points for mRNA half-life assay; RNA levels were detected by qPCR. VEGFA mRNA stability was not affected by knockdown of SCIRT. (i) Cells were treated with ActD and harvested at various time points for mRNA half-lives assay; RNA levels were detected by qPCR. VEGFA mRNA stability was shown to be elevated by overexpression of SCIRT. .

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