Long Noncoding RNA SCIRT Promotes HUVEC Angiogenesis via Stabilizing VEGFA mRNA Induced by Hypoxia

Figure 5

HuR acts as a key downstream factor to induce the angiogenesis effect under HR condition. (a) Inhibition of SCIRT downregulated protein levels of HuR and VEGFA, which was reversed by ectopic expression of HuR. (b) Overexpression of SCIRT upregulated protein levels of HuR and VEGFA, which can be counteracted with HuR knocked down. Protein levels were examined by western blot. Under HR condition, the cell ability of tube formation (c), migration (d), and invasion (e) ability was decreased in HUVECs transfected with SCIRT siRNA, which were rescued by ectopic expression of HuR. On the contrary, the SCIRT overexpression-induced cell angiogenesis ability, evaluated by tube formation assay (f), transwell migration assay (g), and wound-healing assay (h), was hampered by HuR inhibition.

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