lncRNA IGF2-AS Regulates Nucleotide Metabolism by Mediating HMGA1 to Promote Pyroptosis of Endothelial Progenitor Cells in Sepsis Patients

Figure 2

lncRNA IGF2-AS played a regulatory role by binding HMGA1. (a) Starbase predicted binding sites of lncRNA IGF2-AS to HMGA1. (b) RIP experiment verified the binding of lncRNA IGF2-AS to HMGA1. (c, d) qRT-PCR and WB were utilized to measure HMGA1 expression in EPCs of sepsis patients. (e) Pyroptosis of EPCs was detected by TEM. (f, g) qRT-PCR and WB were performed to verify HMGA1 interference efficiency and detect pyroptosis-related factors ASC, Caspase-1, NLRP3, and IL-18 expressions. (h) Expression of HMGA1 was detected by qRT-PCR. vs. Healthy or si-NC.

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