Research Article

Preparation and Multitarget Anti-AD Activity Study of Chondroitin Sulfate Lithium in AD Mice Induced by Combination of D-Gal/AlCl3

Figure 2

Effect of CS-Li on Aβ1-42 aggregation and spatial conformation. (a) ThT fluorescence kinetics of Aβ1-42 aggregation in absence and presence of CS-Li (200 μg/mL, 400 μg/mL) at 37°C for different time (0, 10, 24, 48, 72, and 100 h). (b) Congo red-binding absorption spectra of Aβ1-42 in absence and presence of CS-Li (200 μg/mL, 400 μg/mL) at 37°C for 3 days. (c) ANS fluorescence spectra of Aβ1-42 in absence and presence of CS-Li (200 μg/mL, 400 μg/mL) at 37°C for 3 days. (d) CD spectra of Aβ1-42 in absence and presence of CS-Li (200 μg/mL, 400 μg/mL) at 37°C for 3 days. (e) TEM images of Aβ1-42 in absence and presence of CS-Li (200 μg/mL, 400 μg/mL) at 37°C for 3 days,  nm. (f) Effect of CS-Li on the cell cytotoxicity of Aβ1-42 fibrils. The viability was estimated by MTT assay. ## vs. the control group; , , and vs. the Aβ1-42 group.
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