Protective Effect of Electroacupuncture on the Barrier Function of Intestinal Injury in Endotoxemia through HO-1/PINK1 Pathway-Mediated Mitochondrial Dynamics Regulation

Figure 3

The levels of mRNAs (a, b) and proteins (c, d, e, and f) known as mitochondrial fusion/fission markers and pyroptosis factors in the wild-type Caco-2 cells (a, c, and d) and PINK1-knockout Caco-2 cells (b, e, and f). HO-1: heme oxygenase-1, PINK1: PTEN-induced putative kinase 1, Drp1: the dynamin-related protein 1, Mfn1/2: the mitofusin 1 and 2, Fis1: mitochondrial division protein 1, OpA-1: the optic atrophy 1, IL-1β: interleukin-1β, LPS: lipopolysaccharide, EA + AP: electroacupuncture (EA) performed on acupoint (AP), hemin: a substrate and inducer of HO-1, and ZnPP: zinc protoporphyrin IX, an inhibitor of HO-1, +/-. The cells were or were not treated with corresponding factors. β-Actin served as an internal standard to ensure similar gel loading of the starting material in each sample. The mRNA and protein levels were compared using the paired sample -test. : significant difference compared with the control cells (). ^#: significant difference compared with the LPS-exposed cells ().

(a)

(b)

(c)

(d)

(e)

(f)