lncRNA CRNDE Affects Th17/IL-17A and Inhibits Epithelial-Mesenchymal Transition in Lung Epithelial Cells Reducing Asthma Signs

Figure 6

The lncRNA CRNDE inhibited EMT in human lung epithelial cells via the miR-29a-3p/MCL-1 axis. (a) Target site prediction between miR-29a-3p and MCL-1. (b) qRT-PCR results showing the mRNA levels of miR-29a-3p and MCL-1. (c) Western blot results showing the MCL-1 protein contents. (d) Dual-luciferase reporter assay results showing an association between miR-29a-3p and MCL-1. (e) qRT-PCR results showing the mRNA levels of miR-29a-3p, lncRNA CRNDE, and MCL-1. (f) Western blot results showing the MCL-1 protein abundances. (g) qRT-PCR and western blot results showing mRNA and protein levels of E-cadherin, vimentin, snail, and α-SMA. (h) ELISA results showing levels of inflammatory factors. ^@ vs. NC mimic. ^$ vs. miR-29a-3p mimic+oe-NC. vs. NC mimic+MCL-1 WT. ^# vs. si-NC. ^& vs. si-CRNDE+oe-NC.

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