Research Article

Cryptotanshinone Attenuated Pathological Cardiac Remodeling In Vivo and In Vitro Experiments

Figure 4

CTS treatment inhibited STAT3 and TGF-β/SMAD pathways. (a) Representative Western blots of p-STAT3, T-STAT3, and GAPDH; (b) relative quantification of p-STAT3/T-STAT3; (c) representative Western blots of TGF-β, p-SMAD3, T-SMAD3, p-SMAD2, T-SMAD2, p-SMAD1/5, T-SMAD1/5, and GAPDH; relative quantification of (f) TGF-β/GAPDH, (d) p-SMAD3/T-SMAD3, (e) p-SMAD2/T-SMAD2, and (f) p-SMAD1/5/T-SMAD1/5. (g) All proteins were normalized to corresponding total protein and then normalized to GAPDH before relative quantification (). Data presented as the One-way ANOVA followed by Tukey’s post hoc test was used for examining the significance difference among different groups. Compared with the sham group (); #compared with the AB+VEH group ().
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