Dietary High-Fat Promotes Cognitive Impairment by Suppressing Mitophagy

Figure 7

Ultrastructure synapses. (a) Diagrammatic sketch of a synapse. (b) Using transmission electron microscopy (TEM), we observed that the synaptic structure is clear and intact, and the dense material in the posterior membrane is visible. The morphological structure of the synapse is relatively intact, and no dissolution of the postsynaptic membrane-dense material is observed. Mi: mitochondria; orange arrow: presynaptic membrane; yellow arrow: postsynaptic membrane; blue arrow: synaptic cleft. (c) The synapse density (red circles) displayed no significant difference in two groups. (d, f) Western blotting detected the expression of BDNF, SYN, and PSD95 in vivo (, two-sample -test). (e, g) The expression of BDNF, SYN, and PSD95 in vitro (, one-way ANOVA). All data are expressed as ; , . . Abbreviations: ND: normal diet; HFD: high-fat diet; CON: control; PA: palmitic acid; BDNF: brain-derived neurotrophic factor; PSD95: postsynaptic density protein 95; SYN: synaptophysin.

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