Mitochondrial Dysfunction and Increased DNA Damage in Vascular Smooth Muscle Cells of Abdominal Aortic Aneurysm (AAA-SMC)

Figure 1

(a) Representative images of healthy VSMC ( cultures) and AAA-SMC ( cultures) stained with MitoTracker Red CMXRos. Mitochondrial particle count per cell (b), mitochondrial area (c), mitochondrial perimeter (d), and mitochondrial circularity (e) were determined by ImageJ as described in Materials and Methods. (f) Mitochondrial fission count was calculated using ImageJ data (). (g) The number of mitochondrial DNA copies per cell was determined by real-time PCR. Data points represent the means of each culture (4-6 cells were captured per cell culture) and are shown with median and range. Data were analysed with the Mann–Whitney test; and , ns: not significant.

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