Mitochondrial Dysfunction and Increased DNA Damage in Vascular Smooth Muscle Cells of Abdominal Aortic Aneurysm (AAA-SMC)

Figure 3

ROS production in healthy VSMC and AAA-SMC. (a, b) Analysis of O₂^- production in controls and after 3 h of treatment with 100 nM MitoQ, or 1 μM AngII, or a combination of both. Cells were stained with CellROX green reagent, and fluorescence was measured with a TECAN multiplate reader. Data are shown as mean signal intensity after background subtraction (a) or as absorbance relative to start (b) of three healthy and three AAA-SMC cultures analysed in triplicate. (c, d) Analysis of H₂O₂ amounts in controls and after 24 h of treatment with 1 μM AngII alone or in combination with 100 nM MitoQ. Detection of H₂O₂ was performed with a luminescent ROS-Glo H₂O₂ assay. Data are shown as relative light units (RLU) of three healthy and three AAA-SMC cultures analysed in triplicate and were analysed by multiple Mann–Whitney tests (a, c) or by 2-way ANOVA and Šidák’s multiple comparison test as a post hoc test (b, d). ns: not significant; , , , and .

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