Mitochondrial Dysfunction and Increased DNA Damage in Vascular Smooth Muscle Cells of Abdominal Aortic Aneurysm (AAA-SMC)

Figure 6

AAA-SMCs are subject to earlier growth arrest than healthy VSMCs. Cells were grown with serum withdrawal overnight before full medium was added with or without different concentrations of AngII. (a) Viability at different time points of untreated healthy VSMC and AAA-SMC as determined by WST-1 proliferation assay. Data show the means and ranges of healthy VSMC and AAA-SMC cultures analysed in triplicate. ns: not significant. (b) Apoptosis at different time points of untreated healthy VSMC and AAA-SMC as determined by Caspase-Glo 3/7 Assay. Data show the means and ranges of healthy VSMC and AAA-SMC cultures that were analysed in triplicate. Data were statistically analysed with 2-way ANOVA and Šídák’s multiple comparison test as a post hoc test using the GraphPad Prism software. ; ns: not significant. (c) Upper panel: percentage of p21Waf1/Cip1-positive nuclei in untreated and AngII-treated cell cultures. Data represent the median and range of healthy VSMC and AAA-SMC cultures, of which two or three images were taken for analysis. Data were statistically analysed with unpaired -tests using the GraphPad Prism software. , , and . (c) Lower panel: representative images of samples grown for 72 hours without or with (1 μM) AngII and fluorescently labelled with p21Waf1/Cip1.

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