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Prostate Cancer
Volume 2014, Article ID 129582, 9 pages
Research Article

Role of p73 Dinucleotide Polymorphism in Prostate Cancer and p73 Protein Isoform Balance

1Department of Chemistry, Biochemistry & Physics, University of Tampa, Tampa, FL 33606, USA
2Department of Biostatistics, Moffitt Cancer Center, Tampa, FL 33612, USA
3Department of Cancer Epidemiology, Moffitt Cancer Center, 12902 Magnolia Drive, Tampa, FL 33612, USA
4Department of Molecular Oncology, Moffitt Cancer Center, Tampa, FL 33612, USA
5Department of Anatomic Pathology, Moffitt Cancer Center, Tampa, FL 33612, USA
6Genitourinary Program, James Haley VA Hospital, Tampa, FL 33612, USA
7Genitourinary Program, Moffitt Cancer Center, Tampa, FL 33612, USA

Received 10 March 2014; Revised 21 May 2014; Accepted 22 May 2014; Published 6 July 2014

Academic Editor: Scott E. Eggener

Copyright © 2014 L. Michael Carastro et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Background. Molecular markers for prostate cancer (PCa) risks are currently lacking. Here we address the potential association of a dinucleotide polymorphism (DNP) in exon 2 of the p73 gene with PCa risk/progression and discern any disruption of p73 protein isoforms levels in cells harboring a p73 DNP allele. Methods. We investigated the association between p73 DNP genotype and PCa risk/aggressiveness and survival by fitting logistic regression models in 1,292 incident cases and 682 controls. Results. Although we detected no association between p73 DNP and PCa risk, a significant inverse relationship between p73 DNP and PCa aggressiveness (AT/AT + GC/AT versus GC/GC, OR = 0.55, 95%Cl = 0.31–0.99) was detected. Also, p73 DNP is marginally associated with overall death (dominant model, HR = 0.76, 95%Cl = 0.57–1.00, ) as well as PCa specific death (HR = 0.69, 95%Cl = 0.45–1.06, ). Western blot analyses for p73 protein isoforms indicate that cells heterozygous for the p73 DNP have lower levels of ∆Np73 relative to TAp73 (). Conclusions. Our findings are consistent with an association between p73 DNP and low risk for PCa aggressiveness by increasing the expressed TAp73/∆Np73 protein isoform ratio.