Role of p73 Dinucleotide Polymorphism in Prostate Cancer and p73 Protein Isoform Balance
Figure 2
Western analyses of p73 protein N-terminal isoforms in cancer cell lines. Proteins from cancer cell line lysates in Figure 2(a) (1.25 μg/well) and Figure 2(b) (7.50 μg/well) were resolved on 10% SDS-PAGE gels and the resolved proteins were electrotransferred onto PVDF membranes and then immunoblotted (IB) with a p73 isoform-specific monoclonal antibody against TAp73 or ΔNp73. As a positive loading control, actin was immunoblotted with a goat polyclonal antibody. Primary antibodies were detected using appropriate secondary antibody-horseradish peroxidase conjugates and an enhanced chemoluminescence method followed by fluorography (IB = primary western antibody).
