Inhibition of Prostate Cancer Cells by 4,5-Dicaffeoylquinic Acid through Cell Cycle Arrest

Figure 3

Induction of cell cycle arrest in DU-145 cells by 4,5-diCQA. Cell cycle analysis was performed by setting a threshold for live population based on cell size index and followed by setting gates for the cell cycle based on DNA content. Control group profile (a) was compared to the 4,5-diCQA group treated at the IC₅₀ concentration (b). Majority (about 50%) of the treated cells were in the G0/G1 phase. (c) The percentage of cells in G0/G1 phase decreased (p-value = 0.3717), those in the S phase increased (p-value = 0.0075), and those in G2/M phase did not change significantly (p-value = 0.1726) compared with the control. Data represented are mean SD of four trials.

(a)

(b)

(c)