Inhibition of Prostate Cancer Cells by 4,5-Dicaffeoylquinic Acid through Cell Cycle Arrest

Figure 7

Relative toxicities of 4,5-diCQA on DU-145, LNCaP and PC-3 prostate cancer cells. Experiments were performed by seeding 5000/cells per well in 96-well tissue culture plate. After 24 hours, the culture medium was replaced with media containing 4,5-diCQA for treatment samples. Concentrations of 0.1 μM, 1.0 μM, 2.5 μM, 5.0 μM, and 10.0 μM of 4,5-diCQA were used along with cultures with no treatments (control). After incubation for 72 hours, CCK-8 assay was performed by adding 10% CCK-8 solution in medium followed by incubation at 37°C after which absorbance was measured at 450 nm. Absorbances were normalized to untreated controls to determine cell viability. Mean standard deviation of triplicates is represented.