Interleukin-10 Induces Expression of Neuroendocrine Markers and PDL1 in Prostate Cancer Cells

Figure 2

Inhibition of AR transactivation in IL10, IL6, and ENZ treated cells. LNCaP cells were either untreated (Un) or treated with 100 ng/mL IL10, IL6, or 10 µM ENZ. Cells were collected for flow cytometry analysis at the indicated time points. (a) The gating strategy and how % GFP cells are determined. (b) Histograms are from one of three representative experiments. (c) The % GFP positive cells determined, as shown in panel (a), are plotted for the different treatment groups over time. The statistical significance for the difference between untreated and different treatments was determined by two-way ANOVA test with Tukey’s correction. , , ,and ; ns = not significant.

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