Somatic Mitochondrial DNA Point Mutations Used as Biomarkers to Demonstrate Genomic Heterogeneity in Primary Prostate Cancer

Figure 4

(a) Giemsa stained slice from prostate no. 3, sample 6, transposed mutant fractions obtained by focused laser capture microdissection in a grid of 8 × 12 spots. The red line encircles the tumor as defined by an experienced uropathologist. The circles in (a) show the LCM dissected areas and positions. White represents wild type or homoplasmy. The colors reflect the mutant fractions and their combinations. Corresponding electropherograms for the three circles labeled (A), (B), and (C) are reproduced in (b). (b) Electropherograms related to detected mtDNA mutations, their fractions, and combinations. The red signal represents a fragment specific internal standard used as a reference. Electropherogram (A) reflects mtDNA mutational combinations in the collected spot to the right of the letter (A). Electropherogram (B) reflects mtDNA mutational combinations in the collected spot to the right of the letter (B). Electropherogram (C) reflects mtDNA mutational combinations in the collected spot to the right of the letter (C).

(a)

(b)