Spinal Cord Pathology in Alpha-Synuclein Transgenic Mice

Figure 2

Immunohistochemical analyzes and cell death staining of the cervical spinal cord. Schematic illustration of the transversal spinal cord (a). Cell death staining by FluoroJade and Gallyas are shown in (b) and (c) for BAsyn/PaKO. An example showed a cell positive for Gallyas and GFAP in adjacent slices ((c), (d)). Immunopositive structures of the cervical spinal cord for hm 𝛼-syn (with or without proteinase K), GFAP, Iba1, CATX, and CATS are shown for LM in (e)–(j) and for BAsyn/PaKO in (k)–(p). (q)–(v) are higher magnifications of (k)–(p). Scale bars: (e)–(p): 200 𝜇m; (q) and (r): 10 𝜇m, (s)–(v): 100 𝜇m, and details of (s)–(v): 10 𝜇m.