Phytic Acid Protects against 6-Hydroxydopamine-Induced Dopaminergic Neuron Apoptosis in Normal and Iron Excess Conditions in a Cell Culture Model
Figure 2
Protection of IP6 against 6-OHDA-induced apoptosis in the iron-excess condition in vitro cell culture model. Cell apoptosis was measured as caspase-3 activity (FU/mg protein, (a) from one experiment) with 30 and 100 μmol/L IP6. DNA fragmentation (AU/mg protein, (b) from one experiment) and Hoechst nuclear staining (c) were tested with only 30 μmol/L of IP6. ANOVA with Tukey's Multiple Comparison test was used to detect the differences among the treatments in (a) and (b), and means with different letters were significantly different (). The top panel in C represents 20x magnification and the bottom panel represents 60x magnification. Apoptotic nucleus is indicated with an arrow.
