Effects of Human Alpha-Synuclein A53T-A30P Mutations on SVZ and Local Olfactory Bulb Cell Proliferation in a Transgenic Rat Model of Parkinson Disease
Alpha-synuclein, tyrosine hydroxylase (TH), thioflavin T, and BrdU stainings in wild-type (WT) and transgenic (Tg) rats. Overview of TH expression in wild-type (WT, a) and transgenic (Tg) olfactory bulbs (c). Alpha-syn immunostaining on a section of olfactory bulb of WT (b) and transgenic rat (d). Higher magnification of a Tg glomerular layer stained for TH in green (e), for α-syn in red (f), and with merged stainings (g). Arrows point out some of the many neurons expressing both TH and α-syn (stained in yellow in g). Confocal visualizations in transgenic rat of TH (h), human α-syn (i), and merged TH and α-syn (j). Arrows in h, i, and j point out a TH positive neuron (in green) expressing the human α-syn (in red). (k) Visualization of protein aggregates (arrows) in a Tg glomerular layer using thioflavin T, cell bodies are in red.
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