The Anticholinesterase Phenserine and Its Enantiomer Posiphen as 5′Untranslated-Region-Directed Translation Blockers of the Parkinson’s Alpha Synuclein Expression
Figure 4
Selectivity of posiphen to inhibit translation driven by alpha-synuclein 5′UTR sequences: a second set of transient transfection experiments in which posiphen (10 μM) selectively inhibited alpha-synuclein 5′UTR-conferred luciferase expression in SH-SY5Y neural cells (SNCA 5′UTR-positive transfectants, ()). Confirming selectivity, posiphen increased luciferase expression in pGL3-transfected SH-SY5Y cells (**pGL3-SH-SY5Y serves as an experimental control since these cells were transfected with pGL3, which is the same as the H2A construct but lacks the -synuclein 5′UTR).
