17-Ethynyl-androst-5-ene-3,7,17-triol (HE3286) Is Neuroprotective and Reduces Motor Impairment and Neuroinflammation in a Murine MPTP Model of Parkinson’s Disease
Figure 3
HE3286 attenuated MPTP-induced expression of inflammatory cytokines. Mice (male, C57/Bl6 mice, 8 per group) received four injections of 20 mg/kg MPTP 2 h apart. HE3286 (40 mg/kg), vehicle, or saline was administered by oral gavage twice-daily for four consecutive days beginning 1 h after the last MPTP injection. Gene expression for iNOS (a), TNF-α (b), and IL-1β (c) was assessed by semiquantitative reverse transcriptase-polymerase chain reaction. Gene expression data are presented as the ratio between target gene expression and GAPDH control gene expression. *Statistically significant compared to MPTP + vehicle by the Mann-Whitney test.
