Magnolol Protects against MPTP/MPP⁺-Induced Toxicity via Inhibition of Oxidative Stress in In Vivo and In Vitro Models of Parkinson’s Disease

Figure 5

Effect of MPP⁺ and/or magnolol on mitochondrial REDOX activity in human neuroblastoma SH-SY5Y cells. Cells were treated with 0.5–5.0 mM MPP⁺ for 24 h (a). Cells were treated with 2.5 mM MPP⁺ in the presence or absence of magnolol (1 or 3 μM) for 24 h (b). Mitochondrial REDOX activity was assessed by the Alamar blue assay. Alamar blue was added and cells were incubated for 120 min. These are representative results from at least three independent experiments. Results are means ± SD (𝑛=4). Statistical comparisons were carried out by one-way ANOVA followed by Dunnett’s test. **𝑃<0.01 versus control or vehicle (DMSO), ^#𝑃<0.05 versus MPP⁺ + vehicle (DMSO).

(a)

(b)