A Case-Control Association Study of RANTES (-28C>G) Polymorphism as a Risk Factor for Parkinson’s Disease in Isparta, Turkey

Table 1

Primer sets, melting temperature (MT), PCR product size, and genotyping conditions for IL-8 (-251A>T), MCP-1 (-2518A>G), RANTES (-28C>G), CCR-2 (V64I), and CCR-5 (-Δ32) genes as well as single nucleotide polymorphisms (SNPs).

Gene and polymorphism	Primer sets	MT (°C)	PCR product size (bp)	Restriction enzyme	Homozygote normal (bp)	Homozygote; polymorphic (bp)
IL-8; (−251 A>T)	F: 5′-GATTCTGCTCTTATGCCTCCA-3′	55°C	816 bç	MfeI	296 + 520	810
	R: 5′-CCCAAGCTTGTGTGCTCTGCTGTC-3′
MCP-1; (-2518 A>G)	F: 5′-CCGAGATGTTCCCAGCACAG-3′	60°C	930 bç	PvuII	930	708 + 222
	R: 5′-CTGCTTTGCTTGTGCCTCTT-3′
RANTES; (-28C>G)	F: 5′-ACAGAGACTCGAATTTCCGGA-3′	50°C	187 bç	MnlI	114 + 27 + 20 + 13	134 + 27 + 13
	R: 5′-CCACGTGCTGTCTTGATCCTC-3′
CCR-2 (V64I) (+190G/A)	F: 5′-GGATTGAACAAGGACGCATTTCCCC-3′	63°C	380 bç	FakI	380	215 + 165
	R: 5′-TTGCACATTGCATTCCCAAAGACCC-3′
CCR-5; (-Δ32)	F: 5′-CCTGGCTGTCGTCCATGCTG-3′	57°C	735 bç	EcoRI	403 + 332	371 + 332
	R: 5′-CTGATCTAGAGCCATGTGCACAACTCT-3′