Deletion of Herpud1 Enhances Heme Oxygenase-1 Expression in a Mouse Model of Parkinson’s Disease

Figure 2

Neurodegeneration after MPTP administration. (a, b) Immunohistochemistry. Herpud1^+/+ and Herpud1^−/− mice were injected with MPTP and perfused with 4% paraformaldehyde at the indicated times. Brain sections, including sections with the SNpc (a) or CPu (b), were subjected to immunohistochemistry with anti-TH antibody. The number of TH-positive neurons in the SNpc is shown in (a). Values shown are the mean ± SD (). , compared to mice without MPTP administration. (c) Western blots. Herpud1^+/+ and Herpud1^−/− mice were injected with MPTP, and protein samples (30 μg) extracted from the CPu were subjected to western blotting with anti-TH, anti-GFAP, and anti-β-actin antibodies. The relative intensity of TH is shown in the graph. Each value shown is the mean ± SD (), and the value derived from Herpud1^+/+ mice without MPTP injection is designated as one. , compared to mice without MPTP administration. Scale bars = 100 μm.

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