Review Article

Parkinson’s Disease: The Mitochondria-Iron Link

Figure 2

The iron Chelator M30 protect SH-SY5Y cells from rotenone-induce lipid peroxidation. (a) Mitochondrial lipid peroxidation was evaluated by green/red fluorescence changes of C11-BODIPY581/591 (ThermoFisher Scientific-Molecular Probes) as described [63]. Oxidation of C11-BODIPY581/591 results in a shift of the fluorescence emission peak from 590 nm (red, nonoxidized) to 510 nm (green, oxidized). SH-SY5Y cells were preincubated or not for 24 hours with 500 nM of M30 in DMEM-10% FCS medium and then loaded for 15 minutes at 37°C with 1 μM C11-BODIPY581/591. Confocal images were obtained 15 minutes both before (Control, M30) and after (Rotenone, M30/Rotenone) applying 80 μM rotenone to the cells. Representative images are shown, where the ratio of green over (green + red) fluorescence was converted into a pseudothermal scale using the ImageJ program. (b) Changes in C11-BODIPY581/591 oxidation quantified by the thermal scale. Values represent the mean ± SD of 40–52 individual cell measures per experimental condition. Significance between mean differences was determined by one-way ANOVA and Tukey post hoc test. .
(a)
(b)