Sirtuin-2 Protects Neural Cells from Oxidative Stress and Is Elevated in Neurodegeneration

Figure 5

α-Synuclein aggregate formation and quantification in toxin treated SH-SY5Y cells. SIRT2 overexpressing SH-SY5Y cells were treated with toxin (20 μM or 10 μM diquat or 20 μM or 0.5 μM rotenone) and 0.2% PBS or DMSO; cells transfected with empty vector were used as a control and another set of SIRT2 and control cells was coincubated with AGK2 and toxin. Cells were immunostained with phospho-α-synuclein. Images were captured through GFP filter under 63x magnification. The captured images represent α-synuclein staining and the bar graphs represent the aggregate quantification in diquat or rotenone treated cells. Each bar represents % α-synuclein aggregates (±SD) from three independent assays (). , , and when compared to 0.2% vehicle, one-way ANOVA (Bonferroni corrected), , , and when compared to control cells, , , and when compared to control + AGK2 treatment, and and when compared to SIRT2 cells, two-way ANOVA (Bonferroni corrected). Scale bar: 20 μM.