Oxidized DJ-1 Levels in Urine Samples as a Putative Biomarker for Parkinson’s Disease

<div>Detection of OxiDJ-1 treated with H<sub>2</sub>O<sub>2</sub> in HEK293T cell lysates or cultured media. HEK293T cells were treated with 100 <svg height="9.39034pt" id="M13" style="vertical-align:-3.42943pt" version="1.1" viewbox="-0.0498162 -5.96091 7.34167 9.39034" width="7.34167pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M538 96L524 119C492 88 454 63 446 63C439 63 434 70 440 101C463 223 491 341 518 448H508L433 422L401 276C355 192 240 56 188 56C163 56 154 89 163 133C184 233 207 338 235 443L230 448L152 424L58 17C40 -60 23 -143 23 -185C23 -241 42 -261 63 -261C92 -261 117 -241 125 -227L124 -221C109 -209 89 -165 89 -103C89 -55 96 -13 105 26H107C121 -3 134 -12 151 -12C172 -12 194 -5 221 16C279 61 330 124 384 194H386C381 159 377 141 368 105C343 3 363 -12 383 -12C416 -12 486 35 538 96Z" id="g113-234"></path></g></svg>M H<sub>2</sub>O<sub>2</sub> for 0, 1, and 3 h, and their cell lysates (a) or culture media (d) were subjected to Western blot assay. A quantitative analysis of cellular (b) or media (e) OxiDJ-1 levels, which were normalized by their <i>β</i>-actin (b) or TSG101 (e) levels, was done. 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Parkinson’s Disease

fig2

Figure 2

Figure 2: Oxidized DJ-1 Levels in Urine Samples as a Putative Biomarker for Parkinson’s Disease 