Research Article

Evaluating the Potential of Portulaca oleracea L. for Parkinson’s Disease Treatment Using a Drosophila Model with dUCH-Knockdown

Figure 3

P. oleracea L. extract rescued locomotor dysfunction caused by knockdown of dUCH. (a) Effects of PWE on motion ability of third-instar larvae. Control line TH > GFP-IR (w/y; +; TH-GAL4/UAS-GFP-IR) and dUCH-knockdown line TH > dUCH-IR (w/y; +; TH-GAL4/UAS-dUCH-IR). n = 40; one-way ANOVA, post hoc test: Tukey, ns: not significant, , , , and . Error bars represent the standard deviation of data. (b) Effects of short-term treatment with PWE on climbing ability of adult flies. Short-term treatment: the treatment was interrupted after the third-instar larval stage. Control line (w/y; +; TH-GAL4/UAS-GFP-IR) and dUCH-knockdown line (w/y; +; TH-GAL4/UAS-dUCH-IR). Population size N = 10 and biological replication n = 6; one-way ANOVA, post hoc test: Dunnett, ns: not significant, and . Error bars represent the standard deviation of data. (c) Effects of long-term treatment with PWE on climbing ability of adult flies. Long-term treatment: consecutively treating from embryos to 11-day-old flies. Control line (w/y; +; TH-GAL4/UAS-GFP-IR) and dUCH-knockdown line (w/y; +; TH-GAL4/UAS-dUCH-IR). Population size N = 10 and biological replication n = 6; one-way ANOVA, post hoc test: Dunnett, ns: not significant, , and . Error bars represent the standard deviation of data.
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