Evaluating Dose- and Time-Dependent Effects of Vitamin C Treatment on a Parkinson’s Disease Fly Model

Figure 3

Vitamin C decreased the loss of dopaminergic neurons in dUCH knockdown larvae. Immunostaining images of brain lobes from control (TH-Control) and dUCH knockdown larvae (TH-dUCH KD) using anti-TH antibody. The larvae were treated with different concentrations of vitamin C including 0 mM (A₁−B₁), 0.5 mM (A₂−B₂), and 2.5 mM (A₃−B₃). DM and DL1 clusters are enclosed with white dashed ellipses and scale bars indicate 100 µm. (C) Quantification of dopaminergic neurons in control and dUCH knockdown larvae treated with vitamin C Knockdown of dUCH caused decreases in the numbers of DA neurons in the DM, DL1 clusters, and all of clusters, while the loss of DA neurons in dUCH knockdown larvae was reduced by vitamin C treatment with 0.5 mM and 2.5 mM (one-way ANOVA with Sidak's test, , , and , , error bars indicate the standard deviation of data). TH-Control (+; +; TH-GAL4/+) and TH-dUCH KD (+; +; TH-GAL4/UAS-dUCH.IR).

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