α-Synuclein E46K Mutation and Involvement of Oxidative Stress in a Drosophila Model of Parkinson’s Disease

Figure 1

Human SNCA expression in the brain of transgenic flies. Western blot analyses of the human α-synuclein protein were made on the brain lysates of the flies expressing human SNCA gene via pUAST-GAL4-mediated gene expression. Lane description: marker (M), negative control genotype, (1), UAS-E46K/+ (2), SNCA^wt/+; elav/+ (3), SNCA^E46K/+; elav/+ (4). The human α-synuclein protein was detected using anti-human α-synuclein mouse IgG monoclonal antibody (Invitrogen, USA). An anti-Drosophila ß-actin antibody (Abcam) was used as a control. Gel analysis tool in ImageJ software revealed no significant difference between bands in lanes 3 and 4 (). This indicated equal expression of wild-type and E46K mutant copy of SNCA gene in the brain of the transgenic flies.