Table of Contents
Physiology Journal
Volume 2013 (2013), Article ID 563494, 12 pages
http://dx.doi.org/10.1155/2013/563494
Research Article

Reliability of Calf Bioelectrical Impedance Spectroscopy and Magnetic-Resonance-Imaging-Acquired Skeletal Muscle Hydration Measures in Healthy People

1Graduate Program in Health and Rehabilitation Sciences, Physical Therapy Field, Western University, London, ON, Canada N6G 1H1
2London Health Sciences Center, University Hospital Campus, 339 Windermere Road, London, ON, Canada N6A 5A5
3Western University Division of Nephrology, London Health Sciences Centre, 339 Windermere Road, London, ON, Canada N6A 5A5
4School of Physical Therapy and Department of Epidemiology and Biostatistics, Western University, London, ON, Canada N6G 1H1
5The Centre for Functional and Metabolic Mapping (CFMM), Robarts Research Institute, Western University, 100 Perth Drive, London, ON, Canada N6A 5K8
6School of Physical Therapy, Western University, London, ON, Canada N6G 1H1

Received 3 April 2013; Revised 17 June 2013; Accepted 17 June 2013

Academic Editor: Gary Lopaschuk

Copyright © 2013 Anuradha Sawant et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Purpose. The purpose of this study was to investigate the test-retest reliability, relative variability, and agreement between calf bioelectrical impedance-spectroscopy (cBIS) acquired extracellular fluid (ECF), intracellular fluid (ICF), total water and the ratio of ECF : ICF, magnetic-resonance-imaging (MRI) acquired transverse relaxation times ( ), and apparent diffusion coefficient (ADC) of calf muscles of the same segment in healthy individuals. Methods. Muscle hydration measures were collected in 32 healthy individuals on two occasions and analyzed by a single rater. On both occasions, MRI measures were collected from tibialis anterior (TA), medial (MG), and lateral gastrocnemius (LG) and soleus muscles following the cBIS data acquired using XiTRON Hydra 4200 BIS device. The intraclass correlation coefficients (ICC2,1), coefficient of variation (CV), and agreement between MRI and cBIS data were also calculated. Results. ICC2,1 values for cBIS, , and ADC ranged from 0.56 to 0.92, 0.96 to 0.99, and 0.05 to 0.56, respectively. Relative variability between measures (CV) ranged from 14.6 to 25.6% for the cBIS data and 4.2 to 10.0% for the MRI-acquired data. The ratio of ECF : ICF could significantly predict of TA and soleus muscles. Conclusion. MRI-acquired measures of had the highest test-retest reliability of muscle hydration with the least error and variation on repeated testing. Hence, of a muscle is the most reliable and stable outcome measure for evaluating individual muscle hydration.