Table of Contents
Physiology Journal
Volume 2014 (2014), Article ID 142421, 9 pages
http://dx.doi.org/10.1155/2014/142421
Research Article

Quantitative Analysis of the Relationship between Blood Vessel Wall Constituents and Viscoelastic Properties: Dynamic Biomechanical and Structural In Vitro Studies in Aorta and Carotid Arteries

1Physiology Department, School of Medicine, CUiiDARTE, Republic University, General Flores 2125, 11800 Montevideo, Uruguay
2Favaloro University, C1093AAS Buenos Aires, Argentina
3National Council of Technical and Scientific Research (CONICET), C1033AAJ Buenos Aires, Argentina
4Technological National University, C1179AAQ Buenos Aires, Argentina

Received 26 October 2012; Accepted 11 March 2013; Published 9 April 2014

Academic Editor: Hanjoong Jo

Copyright © 2014 Daniel Bia et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The purposes of this work were to perform in sheep a quantification of the elastic, viscous, and inertial moduli obtained in carotid and aortic artery segments during in vitro dynamic studies that mimic the normal circulatory function; a quantitative determination of collagen, elastin, and vascular smooth muscle of the carotid and aortic segments analyzed in vitro; the correlation between the amounts of each arterial wall constituent and the viscoelastic properties. To this end, nine healthy sheep were included. One artery was selected from each animal to evaluate its biomechanical properties: (a) in three sheep the ascending aorta, (b) in three the thoracic descending aorta, and (c) in the remaining three the proximal segments of the carotid artery. Each selected artery was instrumented with pressure and diameter sensors. After excision, a small ring-shaped sample was set apart from each segment for histological analysis. In conclusion, (a) the arterial compliance showed a positive association with the absolute and relative amount of the parietal elastin, and (b) arterial viscosity was positively associated with the relative amount of smooth muscle, and this association was increased when the correlation was calculated considering the amount of collagen as well as the amount of smooth muscle.