Physiological Responses to Nutrient Accumulation in Trees Seedlings Irrigated with Municipal Effluent in Indian Desert

Table 2

Average values of physiological variables. Values are mean of 12 data (across treatments) for species and 9 data (across species) for treatments.

Species/treatment	Physiological variables
	LWP	𝑃𝑁	𝐸	𝑔s	WUE
Average values across municipal effluent treatments for species
E. camaldulensis	−2.01^a	4.72^a	3.06^a	42.96^a	1.56^b
A. nilotica	−2.20^b	3.82^c	2.63^c	39.04^b	1.44^c
D. sissoo	−1.99^a	4.53^b	2.67^b	42.41^a	1.66^a
Average values across tree species for municipal effluent treatment
T1	−2.28^d	3.35^d	2.05^d	34.45^d	1.57^b
T2	−2.01^b	4.70^b	3.01^b	42.25^b	1.55^b
T3	−1.81^a	5.84^a	3.95^a	50.63^a	1.48^c
T4	−2.15^c	3.52^c	2.13^c	38.56^c	1.62^a
𝐹 with significant level of two-way ANOVA
Species	147.99**	1683.38**	709.21**	61.38**	336.17**
Treatment	335.54**	7552.44**	7335.46**	483.82**	61.14**
Species × treatment	19.81**	86.79**	22.29**	9.16**	25.38**

LWP: leaf water potential (MPa, Mega Pascal); 𝑃_𝑁: rate of photosynthesis 𝑃_𝑁: (𝜇mol CO₂ m⁻² s⁻¹); E: rate of transpiration (mmol m⁻² s⁻¹); 𝑔s: stomatal conductance (×10⁻³ mol m⁻² s⁻¹), and WUE: instantaneous water use efficiency (𝑃_𝑁/𝐸). T₁, T₂, T₃, and T₄ are irrigation of seedlings with municipal effluent at 1/2 PET, 1 PET, 2 PET, and canal water at 1 PET, respectively. ∗∗Significant at 𝑃<0.01. The same letter in the same column means no significant difference (𝑃>0.05) between treatments/species.